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recombinant human vitronectin  (Thermo Fisher)


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    Thermo Fisher recombinant human vitronectin
    Recombinant Human Vitronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+vitronectin/vitronectin++vtn+n++recombinant+human+protein++truncated/pm40595660-230-21-23
    Average 90 stars, based on 1 article reviews
    recombinant human vitronectin - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Restoration of Miro1's N-terminal GTPase function alleviates prenatal stress-induced mitochondrial fission via Drp1 modulation.
    Article Snippet: .. The iPSCs were obtained from the National Stem Cell Bank of Korea and Korean Cell Line Bank (KSCBi005A). iPSCs were cultured at 100,000 cells on plates coated with recombinant human vitronectin (#A14700, Thermo Fisher) and treated with neural induction media (#A1647801, Thermo Fisher) to induce neuronal stem cells (NSCs). .. The cells were replated on dishes coated with Geltrex (#A1413201, Thermo Fisher) and grown in neural differentiation media Neurobasal medium (#21103, Thermo Fisher) supplemented with 2% B27 (#17504, Thermo Fisher) and 1% GlutaMax-1 (#35050, Thermo Fisher) and maintained in a 37 °C, 5% CO2 incubator with humidified atmosphere for 10 days.

    Article Title: Membrane-targeted push-pull azobenzenes for the optical modulation of membrane potential
    Article Snippet: Image analysis was performed using the ImageJ software and the Cell Counter plugin. .. The human induced pluripotent stem cell (hiPSC) line was obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM25256. hiPSCs were cultured on recombinant human vitronectin (rhVTN, ThermoFisher) in E8 Flex medium (ThermoFisher) and differentiated towards hiPSC-derived cardiomyocytes (hiPSC-CMs) on cell culture-grade Matrigel (BD). ..

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids.
    Article Snippet: .. iPSC Culture and Genetic Modification by CRISPR-Cas9 Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Article Title: Restoration of Miro1’s N-terminal GTPase function alleviates prenatal stress-induced mitochondrial fission via Drp1 modulation
    Article Snippet: .. The iPSCs were obtained from the National Stem Cell Bank of Korea and Korean Cell Line Bank (KSCBi005-A). iPSCs were cultured at 100,000 cells on plates coated with recombinant human vitronectin (#A14700, Thermo Fisher) and treated with neural induction media (#A1647801, Thermo Fisher) to induce neuronal stem cells (NSCs). .. The cells were replated on dishes coated with Geltrex (#A1413201, Thermo Fisher) and grown in neural differentiation media Neurobasal medium (#21103, Thermo Fisher) supplemented with 2% B27 (#17504, Thermo Fisher) and 1% GlutaMax-1 (#35050, Thermo Fisher) and maintained in a 37 °C, 5% CO 2 incubator with humidified atmosphere for 10 days.

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids
    Article Snippet: .. Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/ ). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Recombinant:

    Article Title: Restoration of Miro1's N-terminal GTPase function alleviates prenatal stress-induced mitochondrial fission via Drp1 modulation.
    Article Snippet: .. The iPSCs were obtained from the National Stem Cell Bank of Korea and Korean Cell Line Bank (KSCBi005A). iPSCs were cultured at 100,000 cells on plates coated with recombinant human vitronectin (#A14700, Thermo Fisher) and treated with neural induction media (#A1647801, Thermo Fisher) to induce neuronal stem cells (NSCs). .. The cells were replated on dishes coated with Geltrex (#A1413201, Thermo Fisher) and grown in neural differentiation media Neurobasal medium (#21103, Thermo Fisher) supplemented with 2% B27 (#17504, Thermo Fisher) and 1% GlutaMax-1 (#35050, Thermo Fisher) and maintained in a 37 °C, 5% CO2 incubator with humidified atmosphere for 10 days.

    Article Title: Membrane-targeted push-pull azobenzenes for the optical modulation of membrane potential
    Article Snippet: Image analysis was performed using the ImageJ software and the Cell Counter plugin. .. The human induced pluripotent stem cell (hiPSC) line was obtained from the National Institute of General Medical Sciences Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM25256. hiPSCs were cultured on recombinant human vitronectin (rhVTN, ThermoFisher) in E8 Flex medium (ThermoFisher) and differentiated towards hiPSC-derived cardiomyocytes (hiPSC-CMs) on cell culture-grade Matrigel (BD). ..

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids.
    Article Snippet: .. iPSC Culture and Genetic Modification by CRISPR-Cas9 Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Article Title: Restoration of Miro1’s N-terminal GTPase function alleviates prenatal stress-induced mitochondrial fission via Drp1 modulation
    Article Snippet: .. The iPSCs were obtained from the National Stem Cell Bank of Korea and Korean Cell Line Bank (KSCBi005-A). iPSCs were cultured at 100,000 cells on plates coated with recombinant human vitronectin (#A14700, Thermo Fisher) and treated with neural induction media (#A1647801, Thermo Fisher) to induce neuronal stem cells (NSCs). .. The cells were replated on dishes coated with Geltrex (#A1413201, Thermo Fisher) and grown in neural differentiation media Neurobasal medium (#21103, Thermo Fisher) supplemented with 2% B27 (#17504, Thermo Fisher) and 1% GlutaMax-1 (#35050, Thermo Fisher) and maintained in a 37 °C, 5% CO 2 incubator with humidified atmosphere for 10 days.

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids
    Article Snippet: .. Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/ ). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Article Title: Modeling of vascular interactions in endochondral ossification using human embryonic stem cells-derived organoid on a microfluidic chip
    Article Snippet: .. The initial adaptation and maintenance of hESCs were carried out in a commercially available xenofree culture system, utilizing StemFit AK02N medium (#AK02N, Ajinomoto), and dishes were coated with 5 ug/mL recombinant human vitronectin (#A14700, Gibco). ..

    Article Title: Single cell transcriptomics of human kidney organoid endothelium reveals vessel growth processes and arterial maturation upon transplantation.
    Article Snippet: LUMC0072iCTRL01 was obtained from the Leiden hiPSC hotel (approving ethics committee: Leiden University Medical Center Ethics Committee, umbrella protocol 13080, detailed information can be found at https:// npj Regenerative Medicine | (2025) 10:32 9 hpscreg.eu/). iPSC-MAFB was generated from commercially available fibroblasts (ATCC, sourced from third-party suppliers that obtained informed consent). .. Both cell lines were maintained in Essential 8 medium (E8, Thermo Fisher Scientific) with 0.5% Penicillin-Streptomycin (Thermo Fisher Scientific) on recombinant human Vitronectin (Thermo Fisher Scientific). hiPSC-MAFB was used for the scRNAseq experiments and IF stainings. .. LUMC0072 for IF stainingsonly.Cell linesweremycoplasma free. hiPSCs were passaged twice a week using 0.5mM UltraPure EDTA (Thermo Fisher Scientific).

    Modification:

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids.
    Article Snippet: .. iPSC Culture and Genetic Modification by CRISPR-Cas9 Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    CRISPR:

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids.
    Article Snippet: .. iPSC Culture and Genetic Modification by CRISPR-Cas9 Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Generated:

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids.
    Article Snippet: .. iPSC Culture and Genetic Modification by CRISPR-Cas9 Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).

    Article Title: T-Cell Mediated Immune Rejection of Beta-2-Microglobulin Knockout Induced Pluripotent Stem Cell-Derived Kidney Organoids
    Article Snippet: .. Human iPSCs were generated by the Leiden University Medical Center (LUMC) iPSC Hotel using an RNA Simplicon reprogramming kit (Millipore) (LUMC0072iCTRL01, detailed information at Human Pluripotent Stem Cell Registry, https://hpscreg.eu/ ). iPSCs were cultured on recombinant human vitronectin (Thermo Fisher Scientific) coated culture plates in Essential 8 (E8) medium (Thermo Fisher Scientific) and passaged every 3-4 days using 0.5 mM UltraPure EDTA (Thermo Fisher Scientific). .. Genetic modification of iPSCs was performed using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza).



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    <t>Vitronectin</t> Significantly Up‐regulated in CAFs Correlates with Poor Clinical Outcomes in CRC Patients A) Venn diagram demonstrating the intersection of upregulated differentially expressed genes (DEGs) in cancer‐associated fibroblasts (CAFs) compared to normal fibroblasts (NFs), as identified in GSE51257 , GSE92945 , and GSE231559 datasets. B) Heatmap showing the expression patterns of the overlapping genes across the three datasets. C) Violin plots illustrating the expression levels of fibroblast marker genes (ACTA2, COL1A1, COL1A2) and VTN within each cluster, based on a single‐cell RNA sequencing dataset ( GSE231559 ) comprising 6 CRC tissues and 3 adjacent normal tissues. D) tSNE plot of total fibroblasts isolated and re‐clustered from GSE231559 , color‐coded by sample origin (left) and VTN expression (right). E) Representative images of immunofluorescence costaining for VTN (red) with cell‐type markers (green) in CRC tissues from patients. Scale bars: 50 µm. F) Representative images of immunofluorescence co‐staining for VTN (red) with Vimentin, S100A4, and FAP (green) in CRC tissues from patients. Scale bars: 100 µm. G) Representative immunohistochemical staining of VTN in CRC tissue microarray. Magnification: 10X (left) and 200X (right). P: Parenchyma; S: Stroma. H) Immunohistochemical Scoring of VTN in CRC tissues and adjacent normal tissues. Student's t‐test. ***p<0.001. I) Classification of CRC and adjacent tissues into high and low VTN expression groups, followed by Chi‐Square test analysis. p<0.0001, χ2 = 26.97. J) Kaplan‐Meier survival analysis of the impact of VTN expression intensity on progression‐free survival (PFS, left) and overall survival (OS, right) of CRC patients. n = 33 patients for VTN‐low group; n = 47 patients for VTN‐high group. Log‐rank test. p<0.001. K) Higher VTN expression is associated with poorer PFS in The Cancer Genome Atlas (TCGA) CRC cohort (left), and increased distant metastasis rates in the GSE72970 CRC cohort (right). L,M) Verification of CAFs and NFs isolated from CRC tissues and paired noncancerous tissues by immunofluorescent staining (L) and western blot (M) for α‐SMA, Vimentin, FAP, and VTN, with higher expression observed in CAFs compared to NFs. Scale bar: 100 µm. N) qRT‐PCR analysis of VTN mRNA levels in 34 pairs of NFs and CAFs isolated from CRC patients. Paired two‐tailed Student's t‐test was used to calculate p value. P) ELISA detection of VTN expression in plasma samples from CRC patients (n = 36) and healthy individuals (n = 35). Student's t‐test was used to calculate p value. CAFs, cancer‐associated fibroblasts; NF, normal fibroblasts; t‐SNE, t‐distributed stochastic neighbor embedding; CRC, colorectal cancer.
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    86
    Fisher Scientific recombinant human protein vitronectin vtn
    <t>Vitronectin</t> Significantly Up‐regulated in CAFs Correlates with Poor Clinical Outcomes in CRC Patients A) Venn diagram demonstrating the intersection of upregulated differentially expressed genes (DEGs) in cancer‐associated fibroblasts (CAFs) compared to normal fibroblasts (NFs), as identified in GSE51257 , GSE92945 , and GSE231559 datasets. B) Heatmap showing the expression patterns of the overlapping genes across the three datasets. C) Violin plots illustrating the expression levels of fibroblast marker genes (ACTA2, COL1A1, COL1A2) and VTN within each cluster, based on a single‐cell RNA sequencing dataset ( GSE231559 ) comprising 6 CRC tissues and 3 adjacent normal tissues. D) tSNE plot of total fibroblasts isolated and re‐clustered from GSE231559 , color‐coded by sample origin (left) and VTN expression (right). E) Representative images of immunofluorescence costaining for VTN (red) with cell‐type markers (green) in CRC tissues from patients. Scale bars: 50 µm. F) Representative images of immunofluorescence co‐staining for VTN (red) with Vimentin, S100A4, and FAP (green) in CRC tissues from patients. Scale bars: 100 µm. G) Representative immunohistochemical staining of VTN in CRC tissue microarray. Magnification: 10X (left) and 200X (right). P: Parenchyma; S: Stroma. H) Immunohistochemical Scoring of VTN in CRC tissues and adjacent normal tissues. Student's t‐test. ***p<0.001. I) Classification of CRC and adjacent tissues into high and low VTN expression groups, followed by Chi‐Square test analysis. p<0.0001, χ2 = 26.97. J) Kaplan‐Meier survival analysis of the impact of VTN expression intensity on progression‐free survival (PFS, left) and overall survival (OS, right) of CRC patients. n = 33 patients for VTN‐low group; n = 47 patients for VTN‐high group. Log‐rank test. p<0.001. K) Higher VTN expression is associated with poorer PFS in The Cancer Genome Atlas (TCGA) CRC cohort (left), and increased distant metastasis rates in the GSE72970 CRC cohort (right). L,M) Verification of CAFs and NFs isolated from CRC tissues and paired noncancerous tissues by immunofluorescent staining (L) and western blot (M) for α‐SMA, Vimentin, FAP, and VTN, with higher expression observed in CAFs compared to NFs. Scale bar: 100 µm. N) qRT‐PCR analysis of VTN mRNA levels in 34 pairs of NFs and CAFs isolated from CRC patients. Paired two‐tailed Student's t‐test was used to calculate p value. P) ELISA detection of VTN expression in plasma samples from CRC patients (n = 36) and healthy individuals (n = 35). Student's t‐test was used to calculate p value. CAFs, cancer‐associated fibroblasts; NF, normal fibroblasts; t‐SNE, t‐distributed stochastic neighbor embedding; CRC, colorectal cancer.
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    Vitronectin Significantly Up‐regulated in CAFs Correlates with Poor Clinical Outcomes in CRC Patients A) Venn diagram demonstrating the intersection of upregulated differentially expressed genes (DEGs) in cancer‐associated fibroblasts (CAFs) compared to normal fibroblasts (NFs), as identified in GSE51257 , GSE92945 , and GSE231559 datasets. B) Heatmap showing the expression patterns of the overlapping genes across the three datasets. C) Violin plots illustrating the expression levels of fibroblast marker genes (ACTA2, COL1A1, COL1A2) and VTN within each cluster, based on a single‐cell RNA sequencing dataset ( GSE231559 ) comprising 6 CRC tissues and 3 adjacent normal tissues. D) tSNE plot of total fibroblasts isolated and re‐clustered from GSE231559 , color‐coded by sample origin (left) and VTN expression (right). E) Representative images of immunofluorescence costaining for VTN (red) with cell‐type markers (green) in CRC tissues from patients. Scale bars: 50 µm. F) Representative images of immunofluorescence co‐staining for VTN (red) with Vimentin, S100A4, and FAP (green) in CRC tissues from patients. Scale bars: 100 µm. G) Representative immunohistochemical staining of VTN in CRC tissue microarray. Magnification: 10X (left) and 200X (right). P: Parenchyma; S: Stroma. H) Immunohistochemical Scoring of VTN in CRC tissues and adjacent normal tissues. Student's t‐test. ***p<0.001. I) Classification of CRC and adjacent tissues into high and low VTN expression groups, followed by Chi‐Square test analysis. p<0.0001, χ2 = 26.97. J) Kaplan‐Meier survival analysis of the impact of VTN expression intensity on progression‐free survival (PFS, left) and overall survival (OS, right) of CRC patients. n = 33 patients for VTN‐low group; n = 47 patients for VTN‐high group. Log‐rank test. p<0.001. K) Higher VTN expression is associated with poorer PFS in The Cancer Genome Atlas (TCGA) CRC cohort (left), and increased distant metastasis rates in the GSE72970 CRC cohort (right). L,M) Verification of CAFs and NFs isolated from CRC tissues and paired noncancerous tissues by immunofluorescent staining (L) and western blot (M) for α‐SMA, Vimentin, FAP, and VTN, with higher expression observed in CAFs compared to NFs. Scale bar: 100 µm. N) qRT‐PCR analysis of VTN mRNA levels in 34 pairs of NFs and CAFs isolated from CRC patients. Paired two‐tailed Student's t‐test was used to calculate p value. P) ELISA detection of VTN expression in plasma samples from CRC patients (n = 36) and healthy individuals (n = 35). Student's t‐test was used to calculate p value. CAFs, cancer‐associated fibroblasts; NF, normal fibroblasts; t‐SNE, t‐distributed stochastic neighbor embedding; CRC, colorectal cancer.

    Journal: Advanced Science

    Article Title: The Role of CAF‐derived Vitronectin in Promoting Colorectal Cancer Progression and Immunosuppression

    doi: 10.1002/advs.202505769

    Figure Lengend Snippet: Vitronectin Significantly Up‐regulated in CAFs Correlates with Poor Clinical Outcomes in CRC Patients A) Venn diagram demonstrating the intersection of upregulated differentially expressed genes (DEGs) in cancer‐associated fibroblasts (CAFs) compared to normal fibroblasts (NFs), as identified in GSE51257 , GSE92945 , and GSE231559 datasets. B) Heatmap showing the expression patterns of the overlapping genes across the three datasets. C) Violin plots illustrating the expression levels of fibroblast marker genes (ACTA2, COL1A1, COL1A2) and VTN within each cluster, based on a single‐cell RNA sequencing dataset ( GSE231559 ) comprising 6 CRC tissues and 3 adjacent normal tissues. D) tSNE plot of total fibroblasts isolated and re‐clustered from GSE231559 , color‐coded by sample origin (left) and VTN expression (right). E) Representative images of immunofluorescence costaining for VTN (red) with cell‐type markers (green) in CRC tissues from patients. Scale bars: 50 µm. F) Representative images of immunofluorescence co‐staining for VTN (red) with Vimentin, S100A4, and FAP (green) in CRC tissues from patients. Scale bars: 100 µm. G) Representative immunohistochemical staining of VTN in CRC tissue microarray. Magnification: 10X (left) and 200X (right). P: Parenchyma; S: Stroma. H) Immunohistochemical Scoring of VTN in CRC tissues and adjacent normal tissues. Student's t‐test. ***p<0.001. I) Classification of CRC and adjacent tissues into high and low VTN expression groups, followed by Chi‐Square test analysis. p<0.0001, χ2 = 26.97. J) Kaplan‐Meier survival analysis of the impact of VTN expression intensity on progression‐free survival (PFS, left) and overall survival (OS, right) of CRC patients. n = 33 patients for VTN‐low group; n = 47 patients for VTN‐high group. Log‐rank test. p<0.001. K) Higher VTN expression is associated with poorer PFS in The Cancer Genome Atlas (TCGA) CRC cohort (left), and increased distant metastasis rates in the GSE72970 CRC cohort (right). L,M) Verification of CAFs and NFs isolated from CRC tissues and paired noncancerous tissues by immunofluorescent staining (L) and western blot (M) for α‐SMA, Vimentin, FAP, and VTN, with higher expression observed in CAFs compared to NFs. Scale bar: 100 µm. N) qRT‐PCR analysis of VTN mRNA levels in 34 pairs of NFs and CAFs isolated from CRC patients. Paired two‐tailed Student's t‐test was used to calculate p value. P) ELISA detection of VTN expression in plasma samples from CRC patients (n = 36) and healthy individuals (n = 35). Student's t‐test was used to calculate p value. CAFs, cancer‐associated fibroblasts; NF, normal fibroblasts; t‐SNE, t‐distributed stochastic neighbor embedding; CRC, colorectal cancer.

    Article Snippet: The impact of different cell supernatants or recombinant human vitronectin (rhVTN, MedChemExpress, USA) on oxaliplatin‐induced apoptosis in RKO and DLD1 cells was assessed using the annexin V‐FITC/propidium iodide (PI) apoptosis detection kit (Dojindo, Japan) followed by flow cytometry.

    Techniques: Expressing, Marker, RNA Sequencing, Isolation, Immunofluorescence, Staining, Immunohistochemical staining, Microarray, Western Blot, Quantitative RT-PCR, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Clinical Proteomics